1/3/2023 0 Comments Chromatin negative nuclei nbmeThis was achieved by challenging clinically healthy Holstein-Friesian calves (mean age 143 ± 14 days) with either BRSV inoculum ( n = 12) or with sterile phosphate buffered saline (PBS) ( n = 6) and preparing and sequencing ATAC-Seq libraries from fresh BLN tissues. The objectives of this study were to: (i) identify regions of open chromatin in DNA extracted from bronchial lymph nodes (BLN) of healthy dairy calves experimentally challenged with BRSV and compare them with those from non-challenged healthy control calves, (ii) elucidate the chromatin regions that were differentially or uniquely open in the BRSV challenged relative to control calves, and (iii) compare the genes found in regions proximal to the differentially open regions to the genes previously found to be differentially expressed in the BLN in response to BRSV and to previously identified BRD susceptibility loci. DNA-based biomarkers contributing to BRD resistance are potentially present in non-protein-coding regulatory regions of the genome, which can be determined using ATAC-Seq. Bovine Respiratory Syncytial Virus (BRSV) is a cause of Bovine Respiratory Disease (BRD).
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